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polyclonal canine pept1  (Biorbyt)


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    Structured Review

    Biorbyt polyclonal canine pept1
    PCR primers used in real-time PCR assay of transporter expression.
    Polyclonal Canine Pept1, supplied by Biorbyt, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal canine pept1/product/Biorbyt
    Average 90 stars, based on 2 article reviews
    polyclonal canine pept1 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Influence of overexpression of efflux proteins on the function and gene expression of endogenous peptide transporters in MDR-transfected MDCKII cell lines"

    Article Title: Influence of overexpression of efflux proteins on the function and gene expression of endogenous peptide transporters in MDR-transfected MDCKII cell lines

    Journal: International journal of pharmaceutics

    doi: 10.1016/j.ijpharm.2012.12.011

    PCR primers used in real-time PCR assay of transporter expression.
    Figure Legend Snippet: PCR primers used in real-time PCR assay of transporter expression.

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing

    Determination of mRNA levels of endogenous influx transporters, canine PepT1 (cPepT1) and canine PepT2 (cPepT2), and exogenous efflux transporters, human P-gp (hMDR1), human MRP2 (hMRP2) and human BCRP (hBCRP) in various transfected MDCKII cell lines with different passage number using real-time PCR: A, MDCKII-MDR1 cells; B, MDCKII-MRP2 cells; C, MDCKII-BCRP cells. Each bar represents mean ± SD (n = 3). *P < 0.05 and **P < 0.01 compared with the lowest cell passage.
    Figure Legend Snippet: Determination of mRNA levels of endogenous influx transporters, canine PepT1 (cPepT1) and canine PepT2 (cPepT2), and exogenous efflux transporters, human P-gp (hMDR1), human MRP2 (hMRP2) and human BCRP (hBCRP) in various transfected MDCKII cell lines with different passage number using real-time PCR: A, MDCKII-MDR1 cells; B, MDCKII-MRP2 cells; C, MDCKII-BCRP cells. Each bar represents mean ± SD (n = 3). *P < 0.05 and **P < 0.01 compared with the lowest cell passage.

    Techniques Used: Transfection, Real-time Polymerase Chain Reaction

    Western blot analysis of endogenous (canine) PepT2 (A) and PepT1 (B) in wild-type and transfected MDCKII cell lines: lane 1, MDCKII-wt (Pg-14); lane 2, MDCKII-wt (Pg-30); lane 3, MDCKII-MDR1 (Pg-5); lane 4, MDCKII-MDR1 (Pg-30); lane 5, MDCKII-MRP2 (Pg-8); lane 6, MDCKII-MRP2 (Pg-30); lane 7, MDCKII-BCRP (Pg-4); lane 8, MDCKII-BCRP (Pg-30).
    Figure Legend Snippet: Western blot analysis of endogenous (canine) PepT2 (A) and PepT1 (B) in wild-type and transfected MDCKII cell lines: lane 1, MDCKII-wt (Pg-14); lane 2, MDCKII-wt (Pg-30); lane 3, MDCKII-MDR1 (Pg-5); lane 4, MDCKII-MDR1 (Pg-30); lane 5, MDCKII-MRP2 (Pg-8); lane 6, MDCKII-MRP2 (Pg-30); lane 7, MDCKII-BCRP (Pg-4); lane 8, MDCKII-BCRP (Pg-30).

    Techniques Used: Western Blot, Transfection



    Similar Products

    polyclonal canine pept1  (Biorbyt)
    90
    Biorbyt polyclonal canine pept1
    PCR primers used in real-time PCR assay of transporter expression.
    Polyclonal Canine Pept1, supplied by Biorbyt, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal canine pept1/product/Biorbyt
    Average 90 stars, based on 1 article reviews
    polyclonal canine pept1 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    PCR primers used in real-time PCR assay of transporter expression.

    Journal: International journal of pharmaceutics

    Article Title: Influence of overexpression of efflux proteins on the function and gene expression of endogenous peptide transporters in MDR-transfected MDCKII cell lines

    doi: 10.1016/j.ijpharm.2012.12.011

    Figure Lengend Snippet: PCR primers used in real-time PCR assay of transporter expression.

    Article Snippet: Polyclonal canine PepT1 and canine PepT2 primary antibodies were purchased from Biorbyt, Ltd. (Cambridge, Cambridgeshire, UK).

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Determination of mRNA levels of endogenous influx transporters, canine PepT1 (cPepT1) and canine PepT2 (cPepT2), and exogenous efflux transporters, human P-gp (hMDR1), human MRP2 (hMRP2) and human BCRP (hBCRP) in various transfected MDCKII cell lines with different passage number using real-time PCR: A, MDCKII-MDR1 cells; B, MDCKII-MRP2 cells; C, MDCKII-BCRP cells. Each bar represents mean ± SD (n = 3). *P < 0.05 and **P < 0.01 compared with the lowest cell passage.

    Journal: International journal of pharmaceutics

    Article Title: Influence of overexpression of efflux proteins on the function and gene expression of endogenous peptide transporters in MDR-transfected MDCKII cell lines

    doi: 10.1016/j.ijpharm.2012.12.011

    Figure Lengend Snippet: Determination of mRNA levels of endogenous influx transporters, canine PepT1 (cPepT1) and canine PepT2 (cPepT2), and exogenous efflux transporters, human P-gp (hMDR1), human MRP2 (hMRP2) and human BCRP (hBCRP) in various transfected MDCKII cell lines with different passage number using real-time PCR: A, MDCKII-MDR1 cells; B, MDCKII-MRP2 cells; C, MDCKII-BCRP cells. Each bar represents mean ± SD (n = 3). *P < 0.05 and **P < 0.01 compared with the lowest cell passage.

    Article Snippet: Polyclonal canine PepT1 and canine PepT2 primary antibodies were purchased from Biorbyt, Ltd. (Cambridge, Cambridgeshire, UK).

    Techniques: Transfection, Real-time Polymerase Chain Reaction

    Western blot analysis of endogenous (canine) PepT2 (A) and PepT1 (B) in wild-type and transfected MDCKII cell lines: lane 1, MDCKII-wt (Pg-14); lane 2, MDCKII-wt (Pg-30); lane 3, MDCKII-MDR1 (Pg-5); lane 4, MDCKII-MDR1 (Pg-30); lane 5, MDCKII-MRP2 (Pg-8); lane 6, MDCKII-MRP2 (Pg-30); lane 7, MDCKII-BCRP (Pg-4); lane 8, MDCKII-BCRP (Pg-30).

    Journal: International journal of pharmaceutics

    Article Title: Influence of overexpression of efflux proteins on the function and gene expression of endogenous peptide transporters in MDR-transfected MDCKII cell lines

    doi: 10.1016/j.ijpharm.2012.12.011

    Figure Lengend Snippet: Western blot analysis of endogenous (canine) PepT2 (A) and PepT1 (B) in wild-type and transfected MDCKII cell lines: lane 1, MDCKII-wt (Pg-14); lane 2, MDCKII-wt (Pg-30); lane 3, MDCKII-MDR1 (Pg-5); lane 4, MDCKII-MDR1 (Pg-30); lane 5, MDCKII-MRP2 (Pg-8); lane 6, MDCKII-MRP2 (Pg-30); lane 7, MDCKII-BCRP (Pg-4); lane 8, MDCKII-BCRP (Pg-30).

    Article Snippet: Polyclonal canine PepT1 and canine PepT2 primary antibodies were purchased from Biorbyt, Ltd. (Cambridge, Cambridgeshire, UK).

    Techniques: Western Blot, Transfection